The site Lusignan (ICOS code FR-Lus) is a permanent grassland, part of a 160 ha crops and livestock experimental farm operated by INRA since 1970. It is located 3 km south of the city of Lusignan, Vienne, France, Les Verrines site, and 5 km north from the main INRA research center. The site is exposed to oceanic climate, with moderately cold winters and moderately warm summers. The soil is a deep Cambisol with a loamy-clay texture in the upper layers (0-100 cm), and a more heavy clay texture in deeper layers.